SCIENTIST ‘C’ (Microbiology)  
MOLECULAR BIOLOGY


Email: philipicmrnin@gmail.com

Email: philip.abraham@icmr.gov.in

Contact: +91-40-27197236

Brief Profile

Dr. Philip Raj Abraham is a research professional with experience on Tuberculosis from Gulbarga University, Kalaburagi India, ICMR-National JALMA Institute for Leprosy and Other Mycobacterial Diseases Agra (during Ph. D) and DBT-Centre for DNA Fingerprinting and Diagnostics, Hyderabad (Postdoctoral Research Associateships from ICMR and DBT). He also worked for ICMR-National AIDS Research Institute, Pune as Research Scientist/Microbiologist.

He joined ICMR-Vector Control Research Centre (VCRC), Puducherry in July, 2018. He was heading the Unit of Molecular Epidemiology since June, 2021. His research areas were focused on diagnostics and operational research of Vector-Borne Diseases such as Lymphatic Filariasis (LF) and Dengue. Dr. Philip carried out extensive field work in Yadgir- Karnataka, Simdega-Jharkhand, Varanasi – Uttar Pradesh and Balasore – Odisha for filariasis related studies. As a part of the monitoring and evaluation study, he has coordinated the field work to know the prevalence of antigenemia and microfilaremia (funded by BMGF). Further, he has demonstrated that Ineligible population (I-Pop; Pregnant woman, children below 2 years and individual with chronic diseases) and elderly population (E-Pop; individual > 60 years) carry high prevalence of filarial antigen in LF endemic district, Yadgir. Also, he has evaluated the Brugia Rapid Test kits for detection of Brugian filariasis (funded by WHO). Dr. Philip explored the potential application of Dengue-NS1 antigen kits for detection of dengue virus infection in mosquito. He has developed novel assays for dengue such as, Enzyme Linked Lectin Assay (ELLA), Aptamer-Linked Immunosorbent Assay (ALISA), Rabbit Polyclonal antibody Assay for Dengue (RPAD), Human Polyclonal antibody-based Assay for Dengue (HuPA-D), and Human Polyclonal antibody-based Assay for Filariasis (HuPA-F).

He has guided 10 M.Sc. Project students for Dissertation work. He is a recognized Ph. D. supervisor in Microbiology from the Pondicherry University and currently guiding a Ph. D Scholar.

Dr. Philip moved to ICMR-NIN, Hyderabad and currently working at the Molecular Biology Division. His research work is focused on nutritional aspects with respect to Tuberculosis, Filariasis and Dengue, exploring probiotic microorganism for nutrition and molecular studies on food-borne diseases.

Total publications:26
  1. Philip Raj Abraham*, Ramalingam B, Priyadarshini M, Krishnamoorthy K, Hoti S.L, Kumar A. Detection of Wuchereria bancrofti infection in mosquitoes in areas co-endemic with Brugia malayi in Balasore district, Odisha, India. Scientific Reports 14, 16780 (2024). https://doi.org/10.1038/s41598-024-67188-2 (*Corresponding Author)
  2. Philip Raj Abraham*, Tharini Gopinath, Akash Dhotre, Ashwani Kumar. Commercial Enzyme-Linked Immunosorbent Assay Kit is Useful for Detection of Recombinant and Secretory Non-Structural-1 Protein Antigen of Dengue Virus. Vector-Borne and Zoonotic Diseases. 2024 Aug 12. doi: 10.1089/vbz.2024.0009.(*Corresponding Author)
  3. Philip Raj Abraham*, Panneer Devaraju, L. Bharath, Eiriene Prasad, Akash Dhotre, Ashwani Kumar. Evaluation of polyclonal antibodies raised in rabbit against Dengue NS1 antigen. Scientific Reports (Under Revision) (*Corresponding Author)
  4. Muhammed Jabir, B Vijayakumar, K. Krishnamoorthy, Ashwani Kumar, Philip Raj Abraham* Factors influencing participation of elderly population in mass drug administration for lymphatic filariasis: A cross-sectional study. Frontiers in Pharmacology(Under Revision) (*Corresponding Author)
  5. Philip Raj Abraham, Vijesh Sreedhar Kuttiatt  ,  Manju Rahi , Ashwani Kumar. Impediments to compliance during filariasis mass drug administration - observations and recommendations to accelerate filariasis elimination in India. Frontiers in Public Health 2024; 12:1384131. doi: 10.3389/fpubh.2024.1384131.
  6. Ramalingam B, Venkatesan V, Abraham PR, Adinarayanan S, Swaminathan S, Raju KHK, Hoti SL, Kumar A. Detection of Wuchereria bancrofti DNA in wild caught vector and non-vector mosquitoes: implications for elimination of lymphatic filariasis. Mol Biol Rep. 2024 Feb 8;51(1):291.
  7. S. Srivastava, P. R. Abraham , S Mukhopadhyay . Aptamers: An emerging tool for diagnosis and therapeutics in Tuberculosis. Frontiers in Cellular and Infection Microbiology. 2021.(*contributed equally as First Author).
  8. P. R. Abraham , Bharathy R, Pradeep Kumar N , Ashwani Kumar. Dengue NS1 antigen kit shows high sensitivity for detection of recombinant dengue virus-2 NS1 antigen spiked with Aedes aegypti mosquitoes. Scientific Reports 11, 23699 (2021).(*Corresponding Author).
  9. P. R. Abraham, Sankari. T, Pradeep Kumar N, Ashwani Kumar. Detection of recombinant dengue virus 2 NS1 protein in Aedes aegypti mosquitoes using commercial Dengue NS1 ELISA kit. J. Vector Borne Dis. 2022;59(1):98-101.
  10. V.S. Kuttiatt, P. R. Abraham , R.P. Menon, P.C. Vaidya, M. Rahi. COVID-19 in children: clinical and epidemiological implications. Ind. J. Med. Res.2020;152:21-40.
  11. V.S. Kuttiatt, R.P. Menon, P. R. Abraham S. Sharma. Should schools reopen early or late? – Transmission dynamics of COVID-19 in children informs. Ind. J. Pediatrics. 2020; 87:755-756.
  12. G. Pradhan, P. R. Abraham, Shrivastava R, Mukhopadhyay S. Calcium Signaling Commands Phagosome Maturation Process. Int. Rev. Immunol. 2019; 38:57-69.
  13. P. R. Abraham, KP Devalraju, VL Valluri, S Mukhopadhyay . PPE17 (Rv1168c) protein of Mycobacterium tuberculosis detects individuals with latent TB infection. PLoS ONE 2018. 13 (11): e0207787.
  14. P.R Abraham,D Parashar, S Kasetty, V.D. Sharma, C.T. Shivannavar. Characterization of nontuberculous mycobacteria isolated from environmental samples in Kalaburagi district of South India. JSMMicrobiol 2018. 6(1): 1051
  15. P. R. Abraham , N. Pathak, G. Pradhan, G. Sumanlata, S. Mukhopadhyay. The N-terminal domain of Mycobacterium tuberculosis PPE protein Rv1168c plays a dominant role in inducing B-cell response in active TB patients. PLoS ONE 2017. 12(6): e0179965.
  16. P. R. Abraham , A. Udgata, G. Sumanlata, S. Mukhopadhyay. The Mycobacterium tuberculosis PPE protein Rv1168c induces stronger B cell response than Rv0256c in active TB patients. Infect. Genet. Evol. 2016; 40:339-345.
  17. P. R. Abraham , P. Upadhyay, J. Faujdar, G. Ravindranath, S. M. Gaddad, V. D. Sharma, C. T. Shivannavar. Drug susceptibility profiles of Mycobacterium tuberculosis isolates at Gulbarga, South India. Egypt. J. Chest. Dis. Tuberc.2015. 64: 933-937.
  18. P. R. Abraham,, ,G. Sumanlata, V. Valluri, S. Mukhopadhyay. Mycobacterium tuberculosis PPE protein Rv0256c induces strong B cell response in tuberculosis patients. Infect. Genet. Evol. 2014; 22: 244-249.
  19. P. R. Abraham,A. Chauhan, R. Gangane, V. D. Sharma, C. T. Shivannavar. Prevalence of Mycobacterium tuberculosis with multiple copies of IS6110 elements in Gulbarga, South India. Int. J. Mycobact. 2013; 2: 237-39.
  20. P. R. Abraham, V. D. Sharma and C. T. Shivannavar; Diagnosis of TB from smear and culture negative sputum specimens by IS6110 based PCR. Ind. J. Med. Res. 2012:135;249-51.
  21. P. R. Abraham , Sanjay MKasetty,V.D Sharma,Channappa T. Shivannavar. Similarity in the isolation rate of M. tuberculosis for new and treated cases of tuberculosis in sputum specimens preserved under cetylpyridinium chloride. J. Glob. Infect. Dis. 2012; 4: 90-91.
  22. M.R. Thakar,P. R. Abraham, et al., Establishment of reference CD4+ T cell values for adult Indian population. AIDS Res. Ther. 2011:8:35:1-9.
  23. M. K. Sanjay, S. M. Shrideshikan, V. L. Vanishree. M. S. Usha, P. R. Abraham, S. M. Gaddad, C. T. Shivannavar. Copper, Zinc-Superoxide Dismutase from clinically isolated Escherichia coli: cloning, analysis of sodC and its possible role in pathogenicity; Ind. J. Microbiol. 2011; 51 (3)326-331.
  24. A Shete, M. R Thakar, P. R. Abraham and RS Paranjape. A review on peripheral blood CD4+ T Lymphocyte counts in healthy adult Indian: Ind. J. Med. Res. 2010;132 : 667-75.
  25. M. K. Sanjay, S. M. Shrideshikan, M. S. Usha, P. R. Abraham ,S. M. Gaddad, C. T. Shivannavar. Detection, amplification and sequence homology of sodC in clinical isolates of Salmonella sp.Ind. J. Med. Res. 2010; 131: 565-70.
  26. Meyers AFA, et al., QASI:A collaboration for implementation of an independent quality assessment programme in India. Afr J Lab Med. 2016;5(2), a442.
Professional memberships

1. American Society for Microbiology (Global Outreach Contributing Membership level)

2. Indian Association of Applied Microbiologists

3. Society for Vector Ecology (Indian Region)

4. Indian Society of Cell Biology

5. Indian Immunology Society

6. Society of Biological Chemists of India

7. Karnataka Science and Technology Academy

Awards and Honors
  1. Postdoctoral Research Associateships from DBT and ICMR (worked at CDFD, Hyderabad)
  2. “Best Turbo Talk Award” from The National Academy of Vector Borne Diseases (NAVBD) during the 15 th Conference on "Vector and Vector-Borne Disease” held at Goa University, Goa (2023)
  3. Merit scholarship for securing highest marks in Microbiology during under graduation
  4. Travel Grants from DST, ICMR and Prof. G. P. Talwar Travel Bursary for International Conferences at New Orleans, USA and Bangkok, Thailand
  5. Karnataka State Eligibility Test (KSET) for Lecturership (2017)
  6. Recognized as member of Expert Group Committee for finalization of Standard Operating Procedures (SOP’s) and guidelines of Vector Antigen Detection Laboratory at Centre For Medical Entomology & Vector Management (CME & VM) Division, NCDC, Delhi.
  7. Recognized as Doctoral Committee Member for Ph. D Scholars of ICMR-VCRC and Mother Theresa Post Graduate and Research Institute of Health Sciences, Puducherry
  8. Recognized as Member (Microbiologist) of National Joint Outbreak Response Team (NJORT) of ICMR